|
週次 |
日期 |
單元主題 |
|
第1週 |
02/21 |
Overview |
|
第2週 |
02/28 |
[228連假] No class |
|
第3週 |
03/07 |
Generation of Targets for Spotted Microarrays: Inoculate, miniprep, and run DNA gel electrophoresis |
|
第4週 |
03/14 |
Generation of Targets for Spotted Microarrays: PCR intro, spectrophotometer intro |
|
第5週 |
03/21 |
Generation of Targets for Spotted Microarrays: Plate transfer, FX intro |
|
第6週 |
03/28 |
Printing Spotted Glass Microarrays: Printing design (OmniGrid intro) |
|
第7週 |
04/04 |
[溫書假] No class |
|
第8週 |
04/11 |
Printing Spotted Glass Microarrays: Post-processing |
|
第9週 |
04/18 |
[Midterm] No class |
|
第10週 |
04/25 |
Expression Analysis of RNA: Cell culture and RNA extraction |
|
第11週 |
05/02 |
Expression Analysis of RNA: RNA purification and quality check |
|
第12週 |
05/09 |
(Weekend)Expression Analysis of RNA: Probe labeling and array hybridization |
|
第13週 |
05/16 |
(Weekend)Expression Analysis of RNA: Probe labeling and array hybridization |
|
第14週 |
05/23 |
(Weekend)Microarray Bioinformatics: Scan image (Axon scanner intro) and Gal file preparation |
|
第15週 |
05/30 |
Microarray Bioinformatics: Web-based microarray data analysis intro |
|
第16週 |
06/06 |
Microarray Bioinformatics: Real-time RT PCR intro |
|
第17週 |
06/13 |
Microarray Bioinformatics: Illumina and Affymetrix system intro |
|
第18週 |
06/20 |
Final Exam |